Coding
UirR

Part:BBa_K1725420

Designed by: Andrey Filipov   Group: iGEM15_Glasgow   (2015-09-08)

UirR (UV intensity response Regulator)

UirR is a protein part native to the cyanobacterium, Synechocystis. Along with UirS, the proteins form a chromatic two-component system (Sensor and Regulator) to UV and green light (lambda = 534nm) which activates and represses UirS (K1725410), respectively. The protein is attached to the transmembrane protein UirS, which like its other cyanobacteriochrome analogue, ccaS (K608102) is a histidine kinase. After exposure to unidirectional UV-A, UirS releases UirR, which physically interacts with the lsiR promoter (K1725400), activating transcription. All three parts are required for a cell to respond to the UV-A stimulus.

For UirR with a ribosome binding site check K1725421.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 448



References

Ji-Young Song, Hye Sun Cho et al (2011) "Near-UV cyanobacteriochrome signaling system elicits negative phototaxis in the cyanobacterium Synechocystis sp. PCC 6803" PNAS. DOI: 10.1073/pnas.1104242108

Prabha Ramakrishnan† and Jeffrey J. Tabor (2016) "Repurposing Synechocystis PCC6803 UirS–UirR as a UV-Violet/Green Photoreversible Transcriptional Regulatory Tool in E. coli" ACS Synthetic Biology. DOI: 10.1021/acssynbio.6b00068

Yuu Hirose et al (2008)"Cyanobacteriochrome CcaS is the green light receptor that induces the expression of phycobilisome linker protein." Proc Natl Acad Sci U S A.. 105: 9528–9533.

Tabor, J. J. et al.(2010), " Multichromatic Control of Gene Expression in Escherichia coli", J. Mol. Biol. doi:10.1016/j.jmb.2010.10.038

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